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Recombinant Human CD3D & CD3E Heterodimer Protein

Recombinant Human CD3D & CD3E Heterodimer Protein

Item no.	PKSH030479-500ug
Manufacturer	Elabscience
Amount	500 ug
Quantity options	20 ug 50 ug 500 ug
Category	Oncology By Cell Type Hematology Leukemia Lymphoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	Lyophilized Powder
Applications	ELISA
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	FLAG
Purity	> 90 % as determined by reducing SDS-PAGE.
Sequence	Met 1-Ala105&Met 1-Asp126
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD3D, CD3d, T-Cell Receptor T3 Delta Chain, T-Cell Surface Glycoprotein CD3 Delta Chain, T3D
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Proteins / Others
Manufacturer - Conjugate / Tag	C-Flag-hFc
Shipping Temperature	This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight	37.5&40 kDa
Observed Molecular Weight	45-50 kDa
Manufacturer - Research Areas	immunology, Stem cells
Background	T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

PKSH030479-500ug-datasheet.pdf

PKSH030479-500ug-safety-datasheet.pdf