NBL1, Recombinant, Human, aa18-181, His-Tag (Neuroblastoma Suppressor of Tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3)

Blue Ice

20

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.1M NaCl, 10% glycerol, 1mM DTT.

Neuroblastoma suppressor of tumorigenicity 1, also known as NBL1, is a member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. NBL1 is a candidate as a tumor suppressor of neuroblastoma and may play a role in preventing cells from entering the final stage (G1/S) of the transformation process. It is produced in small neurons of the dorsal root ganglion and its expression is activated by MATH-1.

Source:
Recombinant protein corresponding to human NBL1, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~20kD (187aa) (MALDI-TOF)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSAPPPINK LALFPDKSAW CEAKNITQIV GHSGCEAKSI QNRACLGQCF SYSVPNTFPQ STESLVHCDS CMPAQSMWEI VTLECPGHEE VPRVDKLVEK ILHCSCQACG KEPSHEGLSV YVQGEDGPGS QPGTHPHPHP HPHPGGQTPE PEDPPGAPHT EEEGAED

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.