RNASEH2A, Recombinant, Human, aa1-299, His-Tag (Ribonuclease H2 Subunit A, AGS4, JUNB, RNASEHI, RNHIA, RNHL)

Blue Ice

35, 8

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.

Ribonuclease H2 subunit A, also known as RNASEH2A, belongs to the RNase HII family and eukaryotic subfamily. Localized to the nucleus, RNASEH2A mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. RNASEH2A catalyzes the endonucleolytic cleavage of RNA to a 5’-phosphomonoester and is able to bind magnesium or manganese as cofactors. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

Source:
Recombinant protein corresponding to human RNASEH2A, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~35.8kD (322aa)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMDLSELE RDNTGRCRLS SPVPAVCRKE PCVLGVDEAG RGPVLGPMVY AICYCPLPRL ADLEALKVAD SKTLLESERE RLFAKMEDTD FVGWALDVLS PNLISTSMLG RVKYNLNSLS HDTATGLIQY ALDQGVNVTQ VFVDTVGMPE TYQARLQQSF PGIEVTVKAK ADALYPVVSA ASICAKVARD QAVKKWQFVE KLQDLDTDYG SGYPNDPKTK AWLKEHVEPV FGFPQFVRFS WRTAQTILEK EAEDVIWEDS ASENQEGLRK ITSYFLNEGS QARPRSSHRY FLERGLESAT SL

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.