TIM-3, Fc Chimera, Recombinant, Cynomolgus Macaque (T Cell Immunoglobulin and Mucin Domain-3, HAVCR2)

Blue Ice

6075

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with 500ul sterile PBS.

TIM­3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60kD member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like-V-type domain and a Ser/Thr­rich mucin stalk region (1, 2). Mature cynomolgus TIM-3 consists of a 182 amino acid (aa) extracellular domain (ECD), a 21aa transmembrane segment, and a 78aa cytoplasmic tail. Within the ECD, cynomolgus (or crab-eating macaque) monkey TIM-3 shares 81%, 57%, and 56% aa sequence identity with human, mouse, and rat TIM-3, respectively. TIM-3 is up-regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (3). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (6). Soluble TIM­3 is also reported to inhibit the response of T cells to both Ag-induced and concurrent CD3/CD28 stimulation (15). By contrast, TIM-3 interactions with Galectin-9 can trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

Source:
HEK293 ­derived recombinant protein corresponding to Cynomolgus Macaque TIM-3, Fc Chimera

Sequence:
Cynomolgus macaque TIM-3, Ser22-Arg201 (Accession # EHH54703) - IEGRMD - Human IgG1, Pro100-Lys330 (Disulfide-linked homodimer)

Molecular Weight (predicted):
46.3kD (monomer)

Molecular Weight (SDS-PAGE, reducing)
~60-75kD

Activity:
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.5-3ug/ml.

Endotoxin:
<0.10EU/1ug (LAL method).

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.