ATG10, Recombinant, Human, aa1-220, His-Tag (Ubiquitin-like-conjugating Enzyme ATG10, APG10, APG10L, pp12616)

Blue Ice

27, 7

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.

Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome.

Source:
Recombinant protein corresponding to aa1-220 from human ATG10 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~27.7kD (243aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGS
CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP MEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.