CNDP2, Recombinant, Human, aa1-475, His-Tag (Cytosolic Non-specific Dipeptidase, CN2, CPGL, HsT2298, PEPA)

Blue Ice

55, 3

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.

Cytosolic non-specific dipeptidase, also known as CNDP2, is a cytosolic, non-specific dipeptidase that belongs to the peptidase M20A family of proteins. CNDP2 is a secreted peptidase homologous to M20 peptidases. It is expressed by all adult and fetal tissue, however, an isoform lacking exons 3 and 4 was expressed in all fetal tissue, but only in adult liver. Overexpression of CPGL-B in hepatocellular carcinoma cells leads to significant inhibition of HC cell viability, colony formation, cell invasiveness and tumor formation.

Source:
Recombinant protein corresponding to aa1-475 from human CNDP2 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~55.3kD (498aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMAALTTL FKYIDENQDR YIKKLAKWVA IQSVSAWPEK RGEIRRMMEV AAADVKQLGG SVELVDIGKQ KLPDGSEIPL PPILLGRLGS DPQKKTVCIY GHLDVQPAAL EDGWDSEPFT LVERDGKLYG RGSTDDKGPV AGWINALEAY QKTGQEIPVN VRFCLEGMEE SGSEGLDELI FARKDTFFKD VDYVCISDNY WLGKKKPCIT YGLRGICYFF IEVECSNKDL HSGVYGGSVH EAMTDLILLM GSLVDKRGNI LIPGINEAVA AVTEEEHKLY DDIDFDIEEF AKDVGAQILL HSHKKDILMH RWRYPSLSLH GIEGAFSGSG AKTVIPRKVV GKFSIRLVPN MTPEVVGEQV TSYLTKKFAE LRSPNEFKVY MGHGGKPWVS DFSHPHYLAG RRAMKTVFGV EPDLTREGGS IPVTLTFQEA TGKNVMLLPV GSADDGAHSQ NEKLNRYNYI EGTKMLAAYL YEVSQLKD

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.