Stem Cell Factor, Recombinant, Rat (SCF, Kit Ligand, KITLG, Mast Cell Growth Factor, MGF)

Molecular Biology / MB-Growth Factors-Cell Factors

-20°C

Supplied as a lyophilized powder.

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant rat SCF is an 18.4kD polypeptide containing 154aa, which corresponds to the sequence of the secreted soluble form of SCF.

Source:
Recombinant protein corresponding to rat SCF, expressed in E.coli.

Biological Activity:
Determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line. The ED50 was found to be <10ng/ml

Specific Activity:
>1x10e5units/mg

Endotoxin:
<0.1ng/ug (1EU/ug)

AA Sequence:
MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.