Cadherin, Placental (CDHP) Recombinant, Mouse

Blue Ice

28, 1

Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.

CDH3 is a classical cadherin from the cadherin superfamily. The protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Its gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in its gene have been associated with congential hypotrichosis with juvenile macular dystrophy.

Source:
Recombinant Mouse from E. coli

Accession No:
P10287

Fragment:
Lys154~Asp394 (Accession No: P10287)

Sequence:
MGHHHHHHSG SEF-KESGWLL LHMPLDREKI VKYELYGHAV SENGASVEEP MNISIIVTDQ NDNKPKFTQD TFRGSVLEGV MPGTSVMQVT ATDEDDAVNT YNGVVAYSIH SQEPKEPHDL MFTIHKSTGT ISVISSGLDR EKVPEYRLTV QATDMDGEGS TTTAEAVVQI LDANDNAPEF EPQKYEAWVP ENEVGHEVQR LTVTDLDVPN SPAWRATYHI VGGDDGDHFT ITTHPETNQG VLTTKKGLDF EAQD

Epitope Tag:
N-terminal His-Tag

Molecular Weight:
28.1kD

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.
Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.