Carbonic Anhydrase I (CA1) Recombinant, Mouse

Blue Ice

24, 1

Supplied as lyophilized powder in PBS, pH 7.4, 5% sucrose, 0.01% sarcosyl. Reconstitute wiht sterile PBS, pH 7.3.

Recombinant protein corresponding to aa15-223 with a N-terminal His-Tag from mouse expressed in E. coli (P13634)

Amino Acid Sequence:
MGHHHHHHSGS-DQWSKL YPIANGNNQS PIDIKTSEAN HDSSLKPLSI SYNPATAKEI VNVGHSFHVI FDDSSNQSVL KGGPLADSYR LTQFHFHWGN SNDHGSEHTV DGTRYSGELH LVHWNSAKYS SASEAISKAD GLAILGVLMK VGPANPSLQK VLDALNSVKT KGKRAPFTNF DPSSLLPSSL DYWTYFGSLT HPPLHESVTW VICKDSISLS PEQ

Predicted Isoelectric Point:
6.5

Molecular Weight:
24.1kD

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Included Protein Molecular Weight Marker:
168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD
Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.