Cathepsin A (CTSA) Recombinant, Human

Blue Ice

61, 4

Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to aa21-138 with an N-Terminal His Tag from mouse TSHb expressed in E. coli. Swiss/UniProt Accession: P12656.

Amino Acid Sequence:
FCIPTEYTMY VDRRECAYCL TINTTICAGY CMTRDINGKL FLPKYALSQD VCTYRDFIYR TVEIPGCPHH VTPYFSFPVA VSCKCGKCNT DNSDCIHEAV RTNYCTKPQS FYLGGFSV

Predicted Isoelectric Point:
7.6

Predicted Molecular Weight:
14.1kD

Accurate Molecular Weight:
14kD as determined by SDS-PAGE reducing conditions

Subcellular Location:
Secreted

Purity:
>95%

Form:
Supplied as lyophilized powder in PBS, pH7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute in sterile ddH2O. to a concentration of 0.1-0.25mg/ml. Do not vortex.

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.