Complement Component 5a (C5a) Recombinant, Mouse

Blue Ice

9, 7

Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin 300. Reconstitute with sterile PBS, to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to
Asn679~Arg755 of mouse C5a with a N-terminal His-Tag expressed in E. coli. Swiss/UniProt Accession: P06684

Amino Acid Sequence:
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGS-NL HLLRQKIEEQ AAKYKHSVPK KCCYDGARVN FYETCEERVA RVTIGPLCIR AFNECCTIAN KIRKESPHKP VQLGR

Epitope Tag:
N-terminal Tags: His-tag and T7-tag

Molecular Weight:
~9.7kD

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.