Deiodinase, Iodothyronine, Type II (DIO2) Recombinant, Rat

Blue Ice

72, 4

Supplied as lyophilized powder from 10mM PBS, pH 7.4, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin 300. Reconstitute with sterile PBS, to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to Ser30-Phe259 of rat Deiodinase, Iodothyronine, Type II (DIO2) with a N-terminal His tag and MBP-tag expressed in E. coli. Swiss/UniProt Accession: P70551

Amino Acid Sequence:
S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF

Molecular Weight:
72.4kD

Accurate Molecular Mass:
60kD as determined by SDS-PAGE reducing conditions

Phenomenon Explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved
to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.

Predicted Isoelectric Point:
5.8

Subcellular Localization:
Membrane

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.