Fibroblast Growth Factor 10 (FGF10) Recombinant, Rat

Blue Ice

47

Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.

Recombinant proteon corresponding to aa37-215 with an N-Terminal His and GST Tag, expressed in E. coli. Swiss/UniProt Accession: P70492.

Amino Acid Sequence:
QALG QDMVSPEATN SSSSSSSSSS SSSFSSPSSA GRHVRSYNHL QGDVRWRKLF SFTKYFLKIE KNGKVSGTKK ENCPYSILEI TSVEIGVVAV KAINSNYYLA MNKKGKLYGS KEFNNDCKLK ERIEENGYNT YASFNWQHNG RQMYVALNGK GAPRRGQKTR RKNTSAHFLP MVVHS

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.