Podocin (PDCN) Recombinant, Mouse

Blue Ice

30

Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.

Source:
Recombinant Mouse from E. coli

Accession No:
Q91X05

Fragment:
Ile138~Pro371 (Accession No: Q91X05)

Sequence:
MGHHHHHHSGSEF-IFR LGHLLPGRAK GPGLFFFLPC LDTYHKVDLR LQTLEIPFHE VVTKDMFIME IDAVCYYRME NASLLLSSLA HVSKAIQFLV QTTMKRLLAH RSLTEILLER KSIAQDVKVA LDAVTCIWGI KVERTEIKDV RLPAGLQHSL AVEAEAQRQA KVRVIAAEGE KAASESLRMA AEILSGTPAA VQLRYLHTLQ SLSTEKPATV VLPLPFDMLS LLSSPGNRAQ GSINYPSSSK P

Epitope Tag:
N-terminal His-Tag

Molecular Weight:
30kD

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.
Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.