Transmembrane Protease, Serine 2 (TMPRSS2) Recombinant, Human

Blue Ice

27, 2

Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% Trehalose. Reconsitute with sterile dH2O to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to Ser284-Gly492 with an N-terminal His tag from human Transmembrane Protein 2 expressed in E. coli. Swiss/UniProt Accession: O15393.

Amino Acid Sequence:
SIITPEW IVTAAHCVEK PLNNPWHWTA FAGILRQSFM FYGAGYQVEK VISHPNYDSK TKNNDIALMK LQKPLTFNDL VKPVCLPNPG MMLQPEQLCW ISGWGATEEK GKTSEVLNAA KVLLIETQRC NSRYVYDNLI TPAMICAGFL QGNVDSCQGD SGGPLVTSKN NIWWLIGDTS WGSGCAKAYR PGVYGNVMVF TDWIYRQMRA DG

Predicted Isoelectric Point:
8.2

Predicted Molecular Mass:
27.0kD

Accurate Molecular Mass:
27kD as determined by SDS-PAGE reducing conditions

Subcellular Localization:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.