Akr1b1, Recombinant, Mouse, aa1-316, His-Tag (Aldose Reductase)

Blue Ice

38, 1

Supplied as a liquid in PBS, pH 7.4, 10% glycerol.

Akr1b1, also known as Aldose reductase, is an enzyme. It catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

Source:
Recombinant protein corresponding to aa1-316 from mouse Akr1b1, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~38.1kD (339aa), confirmed by MALDI-TOF

Enzyme Activity:
Specific activity is >500 pmol/min/ug, and is defined as the amount of enzyme that catalyzes the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH 7.0 and 37°C.

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.