EEF2K, Recombinant, Human, aa1-725, His-Tag (Eukaryotic Elongation Factor-2 Kinase)

Blue Ice

84, 6

Supplied as a liquid in PBS, pH 7.4, 10% glycerol.

Eukaryotic elongation factor-2 kinase, also known as EEF2K, is threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive.

Source:
Recombinant protein corresponding to aa1-725 from human EEF2K, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~84.6kD (748aa)

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.