ACOT7, Recombinant, Human, aa1-370, His-Tag (Acyl-CoA thioesterase 7)

Blue Ice

42, 6

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.15M NaCl.

ACOT7 is a member of the acyl coenzyme family. This protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this protein may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized.

Source:
Recombinant protein corresponding to aa1-370 of human ACOT7, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~42.6kD

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MARPGLIHSA EEAGAIISTR HCNSQNGERC VAALARVERT DKVLEVPPVV YSRQEQEEEG RKRYEAQKLE VAARHCKTNI VTASVDAINF HDKIRKGCVI TEDEKKRFEE GKGRYLQMKA KRQGHAEPQP PGLPDTCALL QPPAASAAAA PSMSGPDVET PSAIQICRIM RPDDANVAGN VHGGTILKMI DFLSPMCIGE VAHVSAEITY TSKHSVEVQV NVMSENILTG AKKLTNKATL WYVPLSLKNV RMETKWRNGD IVQPVLNPEP NTVSYSQSSL IHLVGPSDCT LHGFVHGGVT MKLMDEVAGI TISGRMTFTS NKSMEIEVLV DADPVVDSSQ KRYRAASAFF TYVSLSQEGR SLPVPQLVPE

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.