CDK2AP1, Recombinant, Human, aa1-115, His-Tag (Cyclin-dependent kinase 2 associated protein 1)

Blue Ice

16, 6

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 50% glycerol.

CDK2AP1 is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. Also, interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes.

Source:
Recombinant protein corresponding to aa1-115 of human CDK2AP1 fused to His-tag expressed in E. coli.

Molecular Weight:
~16.6kD

Amino Acid Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.