Mif, Recombinant, Mouse, aa1-115, His-Tag (Macrophage migration inhibitory factor)

Blue Ice

14, 9

Supplied as a liquid in PBS, 1mM DTT, 10% glycerol.

Mif, also known as Macrophage migration inhibitory factor, has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Source:
Recombinant protein corresponding to aa1-115 of mouse Mif, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~14.9kD

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMPMFIVN TNVPRASVPE GFLSELTQQL AQATGKPAQY IAVHVVPDQL MTFSGTNDPC ALCSLHSIGK IGGAQNRNYS KLLCGLLSDR LHISPDRVYI NYYDMNAANV GWNGSTFA

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.