MRPS2, Recombinant, Human, aa1-296aa, His-Tag (Mitochondrial ribosomal protein S2)

Blue Ice

35, 6

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. MRPS2 is a 28S subunit protein that belongs to the ribosomal protein S2 family.

Source:
Recombinant protein corresponding to aa1-296 of human MRPS2, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~35.6kD

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMATSSAA LPRILGAGAR APSRWLGFLG KATPRPARPS RRTLGSATAL MIRESEDSTD FNDKILNEPL KHSDFFNVKE LFSVRSLFDA RVHLGHKAGC RHRFMEPYIF GSRLDHDIID LEQTATHLQL ALNFTAHMAY RKGIILFISR NRQFSYLIEN MARDCGEYAH TRYFRGGMLT NARLLFGPTV RLPDLIIFLH TLNNIFEPHV AVRDAAKMNI PTVGIVDTNC NPCLITYPVP GNDDSPLAVH LYCRLFQTAI TRAKEKRQQV EALYRLQGQK EPGDQGPAHP PGADMSHSL

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.