PPP3R1, Recombinant, Human, aa1-170, His-Tag (Protein phosphatase 3 regulatory subunit B alpha)

Blue Ice

21, 5

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 0.1M sodium chloride, 10% glycerol.

PPP3R1, also known as Calcineurin subunit B type 1, is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells.

Source:
Recombinant protein corresponding to aa1-170 of human PPP3R1, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~21.5kD

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.