ACE2, Recombinant, Rat, aa18-740, His-Tag (Angiotensin-Converting Enzyme 2)

Blue Ice

84, 7

Supplied as a liquid in PBS, pH 7.4, 10% glycerol.

ACE2, also known as angiotensin-converting enzyme 2, is carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. It is able to hydrolyze apelin-13 and dynorphin-13 with high efficiency and may be an important regulator of heart function.

Source:
Recombinant protein corresponding to aa18-740 from rat ACE2, fused to His-tag at C-terminal expressed in insect cell.

Molecular Weight:
~84.7kD (731aa)

AA Sequence:
QSLIEEKAES FLNKFNQEAE DLSYQSSLAS STGKVCNSMN PQECFLLEPG LDEIMATSTD EQLHAYVRTK LMEVYPSYIS PTGCLPAHLL AWDLGHGDFR IKMCTKVTMD NFLTAHHEMG KWRWMVFQDK IPREQWTKKW WEMKREIVGV LALENVVGSR NMDVKPLLNY FQPLFVWLKE RVSFNFFVTS PKNVSDIIPR SEVEEAIRMS WNYNTNITEE NAQKMNEAAA KWSAFYEEQS KIAQNFSLQE IQNATIKRQL KALQQSGSSA LSPDKNKQLN TILNTMSTIY YNRRLWAWEG WRAEVGKQLR PLYEEYVVLK NEMARANNYE DYGDYWRGDY EAEGVEGYNY NRNQLIEDVE NTFKEIKPLY GDMWGRFWTN LYPLTTPFLQ KPNIDVTDAM VNQSWDAERI FKEAEKFFVS VGLPQMTPGF WTNSMLTEPG DDRKVVCHPT HIQYDMAYAK QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLPSN FQEDNETEIN FLLKQALTIV GTLPFTYMLE VEPLPHDETY CDPASLFHVS NDYSFIRYYT RTIYQFQFQE ALCQAAKHDG PLHKCDISNS TEAGQKLLNM LSLGNSGPWT QNRNSTVGWS TDWSPYADQS IKVRISLKSA LGKNAYEWTD NEMYLFRSSV AYAMREYFSR EKNQTVPFGE ADVWVSDLKP RGRINDIFGL NDNSLEFLGI YPTLKPPYEP PVTLEHHHHH H

Endotoxin: ~1EU/1ug (LAL)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.