AKR1C3, Recombinant, Human, aa1-323 (Aldo-keto reductase family 1 member C3 Isoform 1, DD3, DDX, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, PGFS)

Blue Ice

36, 8

Supplied as a liquid in 20mM Tris-HCl, pH 8.5, 0.1M sodium chloride, 1mM DTT, 10% glycerol.

AKR1C3 also known as Aldo-keto reductase family 1 member C3 isoform 1, is a member of the aldo-keto reductase superfamily which catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9 alpha, 11 beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and differentiation.

Source:
Recombinant protein corresponding to aa1-323 from human AKR1C3, expressed in E. coli.

Molecular Weight:
~36.8kD (323aa)

Specific Activity:
>1000pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1pmole 1-Acenaphthenol in the presence of NADP/minute at pH 8.8 at 25°C.

Amino Acid Sequence:
MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY

Endotoxin: ~1EU/1ug (LAL)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.