BMP-2, Active, Recombinant, Human, aa283-396 (Bone morphogenetic protein 2, BMP-2A)

Blue Ice

13

Supplied as a liquid in 10mM sodium acetate, pH 3.5. No preservative added.

BMP-2 (Bone morphogenetic protein 2) is multi-functional growth factor that belongs to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.

Source:
Recombinant protein corresponding to aa283-396 from human BMP-2, expressed in E. coli.

Molecular Weight:
~13kD (115aa)

Biological Activity:
Measured by its ability to induce alkaline phosphatase production using ATDC5 mouse chondrogenic cells, the ED50 for this effect is <57ng/ml.

Activity Assay:
1. Cell line: ATDC5 (mouse chondrogenic cell)
2. Maintenance Condition: 10% FBS RPMI 1640
3. Assay medium: 1% FBS RPMI 1640
4. Cell Density: 2x10e4 cells/well (96 well plate, final volume 100ul)
5. Starvation: 24hr, Assay medium
6. Incubation Time: 48hr (after sample treatment)
7. Concentration Range: 1ng/ml-10ug/ml
8. Detection method: ALP assay

AA Sequence:
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Endotoxin: ~1EU/1ug (LAL)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.