GPT2, Recombinant, Human, aa1-523, His-Tag(Alanine aminotransferase 2, AAT2, ALT2)

Blue Ice

60, 3

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 2mM DTT, 0.2M sodium chloride, 30% glycerol.

Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney.

Source:
Recombinant protein corresponding to aa1-523 from human GPT2, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~60.3kD (546aa)

Specific Activity:
>100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate/minute at pH 7.5 at 37°C

Activity Assay:
1. Prepare a 180ul assay buffer into a suitable container: The concentrations are 100mM Tris-HCl, 111mM L-Alanine, 0.4mM b-NADH, 6.4mM a-Oxoglutarate, 2.3units/ml LDHA.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer.

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.