PDCD6, Recombinant, Human, aa1-191, His-Tag (Programmed cell death protein 6, ALG-2, PEF1B)

Blue Ice

24

Supplied as a liquid in 10mM sodium citrate, pH 3.5, 40% glycerol.

Programmed cell death 6, also known as PDCD6, is calcium-binding protein belonging to the penta- EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid- induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Recombinant human PDCD6 protein, fused to His-tag at N- terminus, was expressed in E. coli and purified by using conventional chromatography.

Recombinant protein corresponding to aa1-191 from human PDCD6, fused to His-tag at N- terminus, expressed in E. coli.

Molecular Weight:
~24kD (211aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MAAYSYRPGP GAGPGPAAGA ALPDQSFLWN VFQRVDKDRS GVISDTELQQ ALSNGTWTPF NPVTVRSIIS MFDRENKAGV NFSEFTGVWK YITDWQNVFR TYDRDNSGMI DKNELKQALS GFGYRLSDQF HDILIRKFDR QGRGQIAFDD FIQGCIVLQR LTDIFRRYDT DQDGWIQVSY EQYLSMVFSI V

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.