RAP2A, Recombinant, Human, aa1-180, His-Tag (Ras-related protein Rap-2a, K-REV, KREV, RAP2, RbBP-30)

Blue Ice

22, 4

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.

RAP2A, also known as RAP2, is a member of RAS oncogene family and a Ras-like small G protein. This protein shares 60% identity with Rap1A and exhibits a carboxy-terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap1A and Rap1B, overexpression of RAP2A does not interfere with the Ras signaling pathway.

Source:
Recombinant protein corresponding to aa1-180 from human RAP2A, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~22.4kD (200aa)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYEKVP VILVGNKVDL ESEREVSSSE GRALAEEWGC PFMETSAKSK TMVDELFAEI VRQMNYAAQP DKDDPCCSAC

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.