Calbindin D-9K, Recombinant, Rat (D9K, CABP9K, CALB3, CABP1) discontinued

Blue Ice

9

Supplied as a lyophilized powder in 100mM phosphate buffer, pH 7.3.

CABP9K (CALB3 or CABP1; mouse, rat, and human 79 aa; chromosome Xp; ~9 kD) is a cytosolic Ca-binding protein initially found in rat pancreas. It is also expressed in intestine, placenta, uterus and kidney. Its expression is controlled by vitamin D and sex hormone in a tissue specific manner. In keeping with its role in Ca-transport, its expression is highest in duodenal villus enterocytes. It is further shown that CABP9K is only expressed in differentiated enterocytes. CABP9K gene also contains Cdx2-homeoprotein binding sites, and that Cdx2 may play a crucial role in CABP9K transcription. CaBP9K is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Solubility:
It is recommended to reconstitute the lyophilized CABP9K in sterile 18MΩ-cm HO not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized CABP9K although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution CABP9K should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.