Clostripain (Endoproteinase Arg C)

Molecular Biology / MB-Enzymes, Protease (Proteinase, Peptidase)

4°C

Supplied as a dialyzed, lyophilized powder.

Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.

Activity:
≥50 units/mg dry weight

Absorbance (280nm@1mg/ml):
As Reported

Protein:
As Reported

Unit Definition:
1 unit hydrolyzes 1umole of N-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25ºC, pH 7.6, in the presence of 2.5mM dithiothreitol

Quality Control:
SDS-PAGE

Storage and Stability:
Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.