Hepatitis C Virus Core, Nucleocapsid, Recombinant (HCV) (HRP)

Molecular Biology / MB-Infectious Disease

-20°C

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 1mM EDTA, 1.5M urea, 50% glycerol. Labeled with horseradish peroxidase (HRP)

The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways In addition, HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.

The protein contains the HCV core nucleocapsid immunodominant regions.

Specificity:
Immunoreactive with sera of HCV-infected individuals

Applications:
Recombinant HCV Core Antigen may be used in ELISA and Western Blot, excellent for detection of HCV with minimal specificity problems.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.