Hepcidin 25, Mouse, Human, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) Control Peptide

Molecular Biology / MB-Peptides

-20°C

Supplied as a liquid in PBS, pH 7.2

Control peptide for H2008-70 (antiserum) and H2008-72 (affinity purified antibody). Synthetic peptides consisting of 7aa sequence from the N-terminus of mature human and mouse 25aa HEPC were synthesized. he mouse sequences from mouse/rat and human differ by just 1aa in 25aa mature HEPC. The sequence is truncated by 5aa in the 20aa major secreted HEPC. No significant crossreactivity with 20aa HEPC peptide.

Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues. In humans, 20aa and 25aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).

Applications:
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.

Recommended Dilution:
Antibody Blocking: 5-10ug control peptide per 1ul H2008-70 (antiserum) or per 1ug H2008-72 (affinity purified antibody).
ELISA: 1:100, 000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
H2008-73: Hepcidin 25, Mouse, Human, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.