MAD2L1, Recombinant, Human, aa1-205, His-Tag Mitotic Spindle Assembly Checkpoint Protein MAD2A, HsMAD2, Mitotic Arrest Deficient 2-like Protein 1, MAD2-like Protein 1, MAD2)

Blue Ice

25, 7

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT.

MAD2L1, is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate.

Source:
Recombinant corresponding to aa1-205 from human MAD2L1, fused to His-tag at N-terminal expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MALQLSREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG LTLLVTTDLE LIKYLNNVVE QLKDWLYKCS VQKLVVVISN IESGEVLERW QFDIECDKTA KDDSAPREKS QKAIQDEIRS VIRQITATVT FLPLLEVSCS FDLLIYTDKD LVVPEKWEES GPQFITNSEE VRLRSFTTTI HKVNSMVAYK IPVND

Molecular Weight:
~25.7kD (MALDI-TOF)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.