NFNB, Recombinant, E.coli (Oxygen-insensitive NAD(P)H Nitroreductase, Dihydropteridine Reductase, FMN-dependent Nitroreductase, nfnB, dprA, nfsB, nfsI, ntr, b0578, JW0567)

Blue Ice

26

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1mM DTT, 10% glycerol.

NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.

Source:
Recombinant corresponding to aa1-217 of E.coli NFNB protein, fused to His-tag at N-terminus, expressed in E.coli.

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV

Molecular Weight:
26kD (237aa) confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.