Peroxidase, Horseradish (POD, HRP, HRPO) (Biotin)

Molecular Biology / MB-Enzymes

4°C

Supplied as a liquid in PBS , pH 7.4, 1% BSA, 0.1% proclin. Labeled with Biotin and free of unconjugated biotin.

Applications:
Suitable for use in ELISA, Immunohistochemistry, or for antigen applications in immunological protocols. Other applications not tested.

Recommended Dilutions:
P3350-06 (1mg/ml): Use 5ul
Streptavidin (1mg/ml): Use 10ul
10mM PBS, pH 7.4 0.05% Tween-20: Use 25ml
Mix well and incubate for 30 minutes at RT before use.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.