Pin 1, Recombinant, Human (Peptidyl-prolyl cis/trans Isomerase NIMA-interacting 1)

Blue Ice

18, 2

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 5mM DTT, 20% glycerol.

Synonyms: NIMA-interacting Protein 1

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA , PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Sequence:
MADEEKLPPG/ WEKRMSRSSG/ RVYYFNHITN/ ASQWERPSGN/ SSSGGKNGQG/ EPARVRCSHL/ LVKHSQSRRP/ SSWRQEKITR/ TKEEALELIN/ GYIQKIKSGE/ EDFESLASQF/ SDCSSAKARG/ DLGAFSRGQM/ QKPFEDASFA/ LRTGEMSGPV/ FTDSGIHIIL/ RTE

Enyzme Activity: 
Specific activity is > 1, 200 nmoles/min/mg, and is defined as the amount of enzyme that cleaves of suc-AAFP-pNA per minute at 37°C.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.