Phosphatase, Alkaline, Chicken Intestine

Molecular Biology / MB-Enzymes, Phosphatase

4°C

Supplied as a lyophilized powder

Alkaline phosphatase is a broad term associated with non-specific phosphomonesterases with activity optima at alkaline pH.

Activity:
≥0.9 units per mg dry weight (25ºC, pH 8.8)

Unit Definition:
1 unit hydrolyzes 1umole of o-carboxyphenyl phosphate per minute at 25ºC, pH 8.8.

Composition:
The enzyme is a zinc metallo-enzyme. Schüssler (1968) reports four isozymes. Chang and Moog (1972) found three isozymes in the enzyme from chicken duodenum (This preparation is from the whole intestine.)

Optimum pH:
8-9

A280@mg/ml:
As Reported

Activators:
Schüssler (1968) indicates activation by Mg2+. See Sivanaesan et al. (1991).

Inhibitors:
Acidification to pH 4.5 reversibly inactivates the enzyme.

Quality Control:
SDS-PAGE

Storage and Stability:
Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C.