Phosphoenolpyruvate (PEP)

Blue Ice

267, 22

White to off-white crystalline powder

PEP is formed by the action of the enzyme enolase on 2-phosphoglycerate. Metabolism of PEP to pyruvate by pyruvate kinase (PK) generates 1 molecule of adenosine triphosphate (ATP) via substrate-level phosphorylation.

Synonyms:
2-(Phosphonooxy)-2-propenoic acid cyclohexylammonium salt; PEP

CAS No:
10526-80-4

Molecular Formula:
C3H4O6P•C6H14N

Molecular Weight:
267.22 anhy

Appearance:
White to off-white crystalline powder

Purity:
≥97% when determined by enzymatic analysis with pyruvate kinase and lactate dehydrogenase coupling system at pH 7.5.

Free Pyruvate:
≤0.2mole%

Inorganic Phosphorus:
≤0.3 %

Water (KF):
≤4%

Solubility:
Water: 100mg/ml, colorless to faint yellow, clear to very slightly hazy

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.