Phospholipase A2, Secretory, Group IB, Recombinant, Human (PLA2, sPLA2-IB)

Blue Ice

16

Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.

Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentrationdependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.

Description:
The Recombinant Human Secreted Phospholipase A2-IB is manufactured with N-terminal fusionf HisTag. The Recombinant Human Secreted Phospholipase A2-IB His-Tagged Fusion Protein is 16kD containing 126 amino acid residues of the human secreted phospholipase A2-IB and 16 additional amino acid residues-HisTag.

Amino Acid Sequence:
MRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS

Specificity:
The amino acid sequence of the recombinant human Secreted Phospholipase A2-IB is 100% homologous to the amino acid sequence of the human Secreted Phospholipase A2-IB.

Applications:
Western Blot

Reconstitution:
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.

Storage:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.