RND1, Recombinant, Human, aa1-200, His-Tag (Rho-related GTP-binding Protein Rho6, Rho Family GTPase 1, Rnd1, RHO6)

Blue Ice

24, 5

Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 0.1M sodium chloride, 1mM DTT, 30% glycerol.

RND1, also known as Rho 6, is Rho-related GTPases form a distinct branch of the Rho family, since they differ from other Rho proteins in size, charge, and biochemical properties. It appears to be constitutively in the activated GTP-bound form and acts as negative regulators of actin assembly and of cell adhesion. It contributes to regulating the organization of the actin cytoskeleton in response to extracellular growth factors. Purified by using conventional chromatography.

Source:
Recombinant corresponding to aa1-200 of human RND1, fused to His-tag at N-terminal, expressed in E.coli.

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENY TACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETV DSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYE QGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPV

Molecular Weight:
~24.5kD (220aa), confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.