SGK1, Recombinant, Human, aa60-431, His-Tag (SGK, Serum/Glucocorticoid Regulated Kinase 1, Serine/Threonine Protein Kinase SGK1)

Blue Ice

44, 5

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 30% glycerol, 0.2M sodium chloride, 2mM DTT, 0.1mM PMSF.

SGK1, is a serine/threonine protein kinase and a member of the “AGC” subfamily, which includes protein kinases A, G, and C. This protein plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. It is activated in vitroby 3- phosphoinositide-dependent protein kinase-1 (PDK-1) and in vivoin response to signals that activate phosphatidylinositol (PI) 3-kinase.

Source:
Recombinant corresponding to aa60-431 from human SGK1, fused to His-tag at N-terminal expressed in Hi-5 cells using baculovirus expression system and purified by using conventional chromatography techniques..

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPF LVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTV DWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL

Molecular Weight:
~44.5kD

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.