VAMP 2, Recombinant, Human (Synaptobrevin 2, Vehicle-associated membrane)

Blue Ice

13, 8

Supplied as a liquid in PBS, pH 7.4, 1mM EDTA

Synaptobrevin 2 (Vehicle-associated membrane, VAMP2), which is an 18kD integral membrane
protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.

Recombinant protein corresponding to aa1-89 of human VAMP 2, His- tag expressed in E. coli (NP_055047).

Amino Acid Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA TAATAPPAAP AGEGGPPAPP PNLTSNRRLQ QTQAQVDEVV DIMRVNVDKV LERDQKLSEL DDRADALQAG ASQFETSAAK LKRKYW

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, store at -20°C. Aliquots are stable for 6 month after receipts at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.