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Recombinant Yeast Isocitrate Dehydrogenase-1

Recombinant Yeast Isocitrate Dehydrogenase-1

Item no.	enz-289-5mg
Manufacturer	ProSpec
Amount	5mg
Category	Peptides & Proteins Enzymes
Type	Enzymes
Specific against	other
ECLASS 10.1	32160410
ECLASS 11.0	32160410
UNSPSC	12352204
Similar products	iCDH
Available
Synonyms	Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1 1 1 42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD
Introduction	Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+, it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
Description	Recombinant Saccharomyces Cerevisiae ICDH (NADP) derived from yeast host cells by using over-expression system, is full length same as designated ICD1 from Saccharomyces Cerevisiae. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression. The ICDH is purified by proprietary chromatographic techniques.
Source	Yeast cells.
Physical Appearance	Sterile Filtered clear solution.
Formulation	One ml of solution contains 0.075 mol/l KPO₄, 50% Glycerol, pH 7.1.
Purity	Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Biological Activity	The specific activity was found to be 115 U/mg.
Storage	Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

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enz-289-5mg-safety-datasheet.pdf