DNA Polymerase High Fidelity (HiFi) Enzyme

DNA Polymerase (HiFi)

DNA Polymerase High Fidelity (HiFi DNA Polymerase) contains T-DNA Polymerase and a proofreading 3'-5' exonuclease. This kit contains two different buffers: HiFi Buffer 1 is optimized for the amplification of genomic DNA, and HiFi Buffer 2 is optimized for the amplification of ?DNA, cDNA or plasmid DNA. The extension rate is about 1-2 kb/min. Template-independent ""A"" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. Genomic DNA fragments can be amplified up to 15 kb. This kit does not contain 2.5 mM dNTPs.Contents:

Component
Dry Ice	No
Buffer	HiFi DNA Polymerase: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. 10X HiFi Buffer 1/2: 200 mM Tris-HCl (pH 9.0), 100 mM (NH4)2SO4, 20 mM MgSO4, 100 mM KCl, 10% glycerol, other proprietary ingredients.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

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• Product Datasheet
• Safety Datasheet