TopTaq DNA Polymerase Enzyme

TopTaq DNA Polymerase

TopTaq DNA Polymerase contains three binding proteins: one binding protein binds to double-stranded DNA template, preventing polymerase activity at room temperature, and the other two binding proteins bind primers, preventing primer-dimer formation. Together, these binding proteins reduce non-specific amplification. The blocking proteins are released from primers and templates during the initial denaturation. This double-blocking method has higher efficiency and specificity than antibody-based or chemically-modified hot start PCR. Template-independent ""A"" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. This kit does not use Taq antibodies, reducing the risk of mammalian DNA contamination, and this kit does not use chemical modification, so a long denaturing step is not required. Genomic DNA fragments can be amplified up to 15 kb. This kit does not contain 2.5 mM dNTPs.Contents:

Component
Dry Ice	No
Buffer	TopTaq DNA Polymerase: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. 10X TopTaq Buffer: 500 mM Tris-HCl (pH 9.0), 200 mM (NH4)2SO4, 20 mM MgSO4, other proprietary ingredients.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

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