Human AMPK (G1/B2/A1) Heterotrimer Protein

Proteins and Peptides

Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Human AMPK (G1) Protein is a recombinant protein from Human produced in Baculovirus-Insect Cells. A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4.

AAKG1_HUMAN