AceGFast Pfu 2X PCR Master Mix with Dye

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme isolated from Pyrococcus furiosus. Pfu DNA Polymerase possesses 3´?5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. AceGFast Pfu DNA Polymerase has been genetically engineered to enhance the amplification efficiency by fusing DNA binding domains to the Pfu DNA polymerase. AceGFast Pfu DNA Polymerase has the characteristics of high amplification speed (extension ca.2 kb/min), high fidelity and strong anti-interference ability.

Consequently, AceGFast Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. AceGFast Pfu DNA Polymerase-generated PCR fragments are blunt-ended. AceGFast Pfu 2X PCR Master Mix is an optimized ready-to-use solution containing AceGFast Pfu DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ca.4 kb and Tartrazine migrates at ca.10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.