AceG High-Fidelity 2X Master Mix for NGS

AceG High-Fidelity DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. AceG High-Fidelity DNA Polymerase has 5’?3’ polymerase and 3’?5’ exonuclease (proofreading) activity, but no 5’?3’ exonuclease activity. The strong 3’?5’ exonuclease activity results in superior accuracy during DNA amplification, lending to AceG High-Fidelity DNA Polymerase the lowest published error rate of all B-family DNA polymerases (1 error per 3.6 x 106 nucleotides incorporated). This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends.

In AceG High-Fidelity 2X PCR Master Mix with HF Buffer, AceG High-Fidelity DNA Polymerase is supplied in a convenient 2X Master Mix format, containing all reaction components except primers and template. The Master Mix contains AceG High-Fidelity DNA Polymerase (1 U per 50 uL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) and stabilizers. AceG High-Fidelity 2X PCR Master Mix with HF Buffer is designed for routine, high-fidelity PCR of a wide range of targets and fragment sizes. In addition, AceG High-Fidelity DNA polymerase requires significantly shorter reaction times than wild-type B-family DNA polymerases.

DNA fragments generated with AceG High-Fidelity 2X PCR Master Mix may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. PCR products generated with AceG High-Fidelity 2X PCR Master Mix are blunt-ended, but may be 3’-dA-tailed for cloning into TA cloning vectors.