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HARS, 1-509aa, Human, His tag, E.coli

HARS, 1-509aa, Human, His tag, E.coli

Item no.	ARP-01-1818-250
Manufacturer	American Research Products
Amount	250 ug
Quantity options	10 ug 250 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Specific against	other
Purity	> 95 % by SDS - PAGE
Sequence	MGSSHHHHHH SSGLVPRGSH MGSMAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LV
Citations	Raben N., et al. (1992) Nucleic Acids Res. 20(5):1075-81. O'Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294(3):609-14.
NCBI	NP_002100
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic
Available
Description	HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Form	Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Storage	Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms	FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic
Concentration	1 mg/ml (determined by Bradford assay)
NCBI	NP_002100
References	Raben N., et al. (1992) Nucleic Acids Res. 20(5):1075-81. O'Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294(3):609-14.
Antigen Recognized in Species	Human
Purity	>, 95 % by SDS - PAGE
Molecular Weight	59.4 kDa (532aa), confirmed by MALDI-TOF
Amino Acid Sequence	MGSSHHHHHH SSGLVPRGSH MGSMAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC
Gene Category	Enzymes

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 250 ug

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Delivery expected until 7/17/2025

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