KIR2DL1(p58 KIR, CD158), Human, Recombinant, Recombinant, E.coli

An inhibitory Keller Cell Ig-like Receptor(KIR, previously called p58 KIR, p58.1, cl-42, NKAT1, or KIR-K6), which recognizes class I MHC molecules(HLA-Cw2, -Cw4, -Cw5, and Cw6). The protein coding region of the extracellular domain of KIR2DL1(amino acids 1-202) was cloned into an E. coli expression vector. The extracellular domain of KIR2DL1 was overexpressed as insoluble protein aggregates(inclusion bodies). The recombinant KIR2DL1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

1 mg/ml (determined by Bradford assay)

• Steffens, U. et al. (1998) Tissue Antigens 51, 398-413.
• Wagtmann, N.R., et al. (1995) Immunity 2, 439-449.
• Colonna, M. et al. (1995) Science 269, 405-408,
• Kim, J. et al. (1997) J. Immunol. 159, 3875-3882.

>, 95% by SDS - PAGE

MEGVHRKPSL LAHPGRLVKS EETVILQCWS DVMFEHFLLH REGMFNDTLR LIGEHHDGVS KANFSISRMT QDLAGTYRCY GSVTHSPYQV SAPSDPLDIV IIGLYEKPSL SAQLGPTVLA GENVTLSCSS RSSYDMYHLS REGEAHERRL PAGPKVNGTF QADFPLGPAT HGGTYRCFGS FHDSPYEWSK SSDPLLVSVT GN